1. I assume you still have IIS running on Port 8080 on the internal NIC IP . Check whether it is working to connect to you site on port 8080.
This will make sure your website is not using port 80 still.
2. In ISA, do you have auto discovery turned on? If so , by default it will use port 80 and can conflict with your web proxy port
3. Do you have any web publishing rule created in your ISA that use a Web listener to listen on port 80 on the internal NIC as well?
Bala Natarajan [MSFT]| Sr. Support Escalation Engineer | CSS Security -TMG Beta support team |
Email: bala.natarajan@microsoft.com | Office 425.704.4626
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