Neelam ,
Please go through the documentation of various classes that BizTalk RFID provide from our chm file.
RfidCommon and ProcessManagerProxy will help you to achieve the above.
Also there is something called DeviceSimulator which simulates RFID devices. Info about device simulator is available in our documentation chm file.
string fileName = @"Tre1.xml";
FileStream stream = new FileStream(fileName,FileMode.Open,FileAccess.Read);
TagReadEvent tre = RfidCommon.DeserializeFromXmlDataContract(stream, typeof (TagReadEvent)) as TagReadEvent;
ProcessManagerProxy pmp = new ProcessManagerProxy();
pmp.AddEventToProcessPipeline("MyProcess",tre,"MyLogicalDeviceName");
Thanks,
Srujan.