Hi,
Thank you for the post.
According to your description, you have deployed Back to Back Firewall setup and ISA is your proxy server. If anything misunderstand, please let me know.
this issue may related to ISA configuration error. Please refer to the following article to check your settings.
http://www.isaserver.org/tutorials/Configuring-Domain-Members-Back-to-Back-ISA-Firewall-DMZ-Part2.html
Meanwhile, please look into the ISA logging when the client access the website. And you can find whether there is something block by certain policy.
Regards,
Nick Gu - MSFT