You might check out the guidance from Microsoft around this. It isn't step by step but should get you on the right track.
http://technet.microsoft.com/en-us/appvirtualization/cc843994.aspxThere is a whitepaper called "
Virtual Application Management with Microsoft Application Virtualization 4.5 and System Center Configuration Manager 2007 R2" that has all the basic principals.
As mentioned by Kent, when you use SCCM R2 as your distribution method no App-V Servers are required. Only the sequencer for creating packages and the client installed in standalone mode are required.
The rest is handled by SCCM R2.
Happy Sequencing
mattmcdermott