I have run into a random issue of the following error.

I have virtualized a few packages in App-V 5.0, they are connected via Connection Group, using remote app to the Virtual application. (Although I can log directly into the server with that user and run from the globally published icon and get the same
error, so I know that is been ruled out)
I created a new user with the correct permissions, was able to open one time, close the app and get the same error.
I have found that in the past when I get this error I could delete the Local and roaming profiles and the problem would go away. I tried that and the error still arises.
I have remove the application from the publishing server and re-added and no issue
Some notices:
Even though I have closed the application, the Non Admin user will stay disconnected when looking at the RDS Manager
I have added the same user as a domain admin and still get the error directly from the Datacenter client where the application is published
I can log the user off via the RDS Manager and try again. Still same error
RDS Host settings are set to End disconnected session after 1 minute, same issue
I get this same error in the event viewer:
The virtual application '\Device\HarddiskVolume2\ProgramData\App-V\896E6972-6470-437C-A045-9AD7270A13B9\E5ACD190-CDEF-44AD-965B-85797C6BE5F1\Root\VFS\APPVPACKAGEDRIVE\MOMLOCAL8\momwin.exe' could not be launched because the App-V Client could not determine
which environment to use. Provide a priority to the app connection group for the package and try again.
Paul
Duramaxster